His-tag Peptide 
Datasheet
COA
MSDS
SHIP
Product Name :
His-tag Peptide Applications :
Blocking Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Alternative Name :
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag Swiss-Prot :
N/A Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide His-tag. (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using AP0032 His-tag pAb.
His-tag Peptide Applications :
Blocking Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Alternative Name :
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag Swiss-Prot :
N/A Product :
1 mg/ml in DI water. Purification&Purity :
Synthetic peptide His-tag. (Note: the amino acid sequence is proprietary). The purity is > 98%. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This peptide can be used with studies using AP0032 His-tag pAb.
Blocking peptide available as AP0032PP