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Product Catalog
p16 (A20) pAb
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M | 100μg | WB |  |
258.00 |  |
|
Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 16 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p12 INK/p16 INK4 (A20) pAb detects endogenous levels of p12 INK/p16 INK4 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000
Western Blot(WB)
Western blot (WB) analysis of p12 INK/p16 INK4 (A20) pAb in extracts from HeLa cells.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The melanoma susceptibility locus cyclin-dependent kinase inhibitor 2A encodes two unrelated cell growth inhibitors, p16 and alternative reading frame (ARF). It acts as a negative regulator of the proliferation of normal cells, a key barrier to tumor evelopment, by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.The p16 coding sequence is more often mutated in melanoma families than is the ARF sequence.