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Product Catalog
p-Src (Y418) pAb
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Download PDF Datasheet
Catalogue No : BS4176
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 60 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-Src (Y418) pAb detects endogenous levels of Src protein when phosphorylated at Tyr418.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of p-Src (Y418) pAb in extracts from COLO205 cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of p-Src (Y418) pAb in paraffin-embedded human colon carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Nuclear receptors for steroids, thyroid hormones and retinoic acids are liganddependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormoneresponsive elements. This family includes GRIP1 (for GR interacting protein 1, also designated NCoA-2 or Tif2); SRC-1 (for steroid receptor coactivator-1, also designated NCoA-1); RAC3 (also designated AIB1, for amplified in breast cancer, or ACTR), which displays elevated expression in estrogen receptor positive ovarian and breast cancers; and p/CIP (for p300/CBP/co-integrator protein), which is required for the transcriptional activation of p300/CBPdependent transcription factors.