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Product Catalog
p-HSF1 (S303) Antibody
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Download PDF Datasheet
Catalogue No : BS4795
| Host | Reactivity | Size | Application | stock | price | cart |
| BS4795 | H,M | 100μg | WB IHC IF |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-HSF1 (S303) antibody detects endogenous levels of p-HSF1 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IF:1:50~1:200
Western Blot(WB)
Western blot (WB) analyzes of p-HSF1 (S303) antibody in extracts from A549 PMA cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of p-HSF1 (S303) antibody in paraffin-embedded human brain tissue.
Storage&Stability
Store at 4
Background
Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway. Phosphorylation of HSF1 on Ser 230 by heat shock has been shown to positively contribute to the transcriptional activity of HSF1.