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Product Catalog
p-Catenin-β (T41+S45) pAb
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Download PDF Datasheet
Catalogue No : BS4030
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB |  |
298.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 85 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-Catenin-β (T41+S45) pAb detects endogenous levels of Catenin-β protein only when phosphorylated at T41+S45 .
Applications (Recommended Dilutions)
WB: 1:500~1:1000
Western Blot(WB)
Western blot (WB) analysis of p-Catenin-β (T41+S45) pAb in extracts from sw626 cells.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The catenins, α, β and γ, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. β-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. β-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3β, results in accumulation of the β-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of β-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in β-catenin, inhibiting the interaction with APC, and increasing β-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of β-catenin in tumors such as breast cancer.