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Product Catalog
p-CPI-17 (T38) Antibody
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Download PDF Datasheet
Catalogue No : BS4780
| Host | Reactivity | Size | Application | stock | price | cart |
| BS4780 | H,M,R | 100μg | WB IHC |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-CPI-17 (T38) antibody detects endogenous levels of p-CPI-17 antibody protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of p-CPI-17 (T38) antibody in extracts from RAW264.7 cells .
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of p-CPI-17 (T38) antibody in paraffin-embedded human heart tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle Myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonin 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle cont-raction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase."