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Product Catalog
p-Bcr (Y177) pAb
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Catalogue No : BS4026
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M | 100μg | WB |  |
298.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 160 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-Bcr (Y177) pAb detects endogenous levels of Bcr protein only when phosphorylated at Y177.
Applications (Recommended Dilutions)
WB: 1:500~1:1000
Western Blot(WB)
Western blot (WB) analysis of p-Bcr (Y177) pAb in extracts from 293 cells treated with Forskolin
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The Bcr gene, mapping on chromosome 22, was initially identified on the basis of its fusion with the c-Abl proto-oncogene on chromosome 9 resulting in the generation of the Philadelphia chromosome in 90-95% of patients with chronic myelogenous leukemia (CML). The Bcr gene encodes for the Breakpoint cluster region protein (Bcr). A consequence of this translocation is the generation of a Bcr/c-Abl mRNA encoding an activated c-Abl protein kinase. The Bcr gene has been shown to encode a 160 kDa GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, Rac 1 p21. While it has been speculated that the Bcr protein may also stimulate Rac 2 p21 GTPase activity, it has no effect on Ras p21 or Rho p21 GTPases. It is of interest that the GAP domain of Bcr maps outside of the region that remains