Email:shdiahds@163.com
Phone:+1-909-839-7620
Fax:+1-909-839-7620
Click here for qusetions for this product!
Product Catalog
p-ATF2 (T71/53) pAb
Current Country :
Download PDF Datasheet
Catalogue No : BS4018
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC IP |  |
298.00 |  |
|
Contact information
|
||||||
Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 52 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
p-ATF2 (T71/53) pAb detects endogenous levels of ATF2 protein only when phosphorylated at T71/53.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IP:1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of p-ATF2 (T71/53) pAb in extracts from HeLa cells treated with UV..
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of p-ATF2 (T71/53) pAb in paraffin-embedded human breast carcinoma tissue
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular CIS acting promotor and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA. Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family.