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Product Catalog
flag-tag mAb
| Host | Reactivity | Size | Application | stock | price | cart |
| Mouse | All | 100μg | WB,IF |  |
258.00 |  |
|
Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Molecular Weight
Purification & Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
flag-tag mAb detects over-expressed or recombinant proteins containing the flag epitope tag.
Applications (Recommended Dilutions)
WB:1:500-1:2000 IF:1:50-1:200
Western Blot(WB)
Western blot (WB) analyzes of flag-tag mAb in extracts from HUVEC cells transfected with flag -tagged vector.
Immunofluorescence (IF)
Immunofluorescence (green) analyzes of flag-tag mAb in HUVEC cells transfected with flag-tagged vector.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.