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Product Catalog
TRP2 (K89) Antibody
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Download PDF Datasheet
Catalogue No : BS3320
| Host | Reactivity | Size | Application | stock | price | cart |
| BS3320 | H,M | 100μg | WB IF |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
TRP2 (K89) antibody detects endogenous levels of TRP2 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IF: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of TRP2 (K89) antibody in extracts from K562 cells.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme,is involved in the production of melanin, the primary pigment found in vertebrates.Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteinerich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exist as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.