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Product Catalog
Recombinant Human MIP-3/CCL23
(rHu MIP-3/CCL23)
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| Catalogue No | Size | Format | stock | price | cart | |
| PR1097 | 1mg | None |  |
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Source:
Escherichia coli.
Molecular Weight:
11.3 kDa, a single, non-glycosylated polypeptide chain containing 99 amino acids.
Purity:
>97% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. Determined by its ability to chemoattract human T cell population using a concentration range of 10.0 -50.0 ng/ml, corresponding to a Specific Activity of ＞2 x 104 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH 7.4, 150mM NaCl.
AA Sequence:
RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESYFETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
Endotoxin:
Less than 1EU/mg of rHuMIP-3/CCL23 as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage:
This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China
Description:
MIP-3/CCL23 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Alternative splicing of the MPIF1 gene results in two mRNAs that encode a short (CKβ8) and a long (CKβ81) isoform of the chemokine. CKβ8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CKβ8 (aa 22 120). Additional N terminal processing of the 99 aa residue variant can generate a 75 aa residue CKβ8 (aa 46 120) that is significantly more active than the 99 aa residue variant.