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Product Catalog
Histone H4 (S1) pAb
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Catalogue No : BS1663
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC IF |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 11 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Histone H4 (S1) pAb detects endogenous levels of Histone H4 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IF:1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of Histone H4 (S1) pAb in extracts from COS7 cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of Histone H4 (S1) pAb in paraffin-embedded human breast carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.