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Product Catalog
Histone H3 (T22) pAb
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Catalogue No : BS1405
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 20 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Histone H3 (T22) pAb detects endogenous levels of Histone H3 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of Histone H3 (T22) pAb in extracts from HuvEc cells treated with Serum 20% 30'.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of Histone H3 (T22) pAb in paraffin-embedded human breast carcinoma .
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.