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Product Catalog
HES-6 (A6) pAb
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H | 100μg | WB IHC |  |
258.00 |  |
|
Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 30 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
HES-6 (A6) pAb detects endogenous levels of HES-6 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of HES-6 (A6) pAb in extracts from HeLa cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of HES-6 (A6)pAb in paraffin-embedded human brain tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The Drosophila hairy and enhancer of split genes encode basic helix-loophelix (bHLH) transcriptional repressors that function in the notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homologues of Drosophila hairy and enhancer of split are the HES gene family members, HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homologue of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N-box (CACNAG) as opposed to the E-box (CANNTG). HES2 binds to both N- and E-box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.