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Product Catalog
Cytokeratin 18 (P27) pAb
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Download PDF Datasheet
Catalogue No : BS1205
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC IF IP |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 47 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Cytokeratin 18 (P27) pAb detects endogenous levels of Cytokeratin 18 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of Cytokeratin 18 (P27) pAb in extracts from HeLa cell treated with UV 5'.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of Cytokeratin 18 (P27) pAb in paraffin-embedded human breast carcinoma tissue .
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Cytokeratins comprise a diverse group of intermediate filament proteins(IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.