Product

Luminol Reagent,10ml
Peroxide Solution, 10ml

Purification & Purity

Biodlight Western HRP Substrate provides high sensitivity in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose transfer membranes,and is compatible with all commonly used buffers and blocking reagents.The HRP substrate consists of Luminol Reagent and Perixide Solution.Working HRP substrate is prepared by combining equal volumes of Luminol Reagent and Perixide Solution.The HRP substrate produces a high intensity signal with low background for detection of both high and low abundance.

Specificity

Applications (Recommended Dilutions)

No dilution need.

Storage&Stability

Store at 2~8°C.

Background

Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates.The speed and sensitivity of these methods are unequalled by traditional alternatives. Horseradish peroxidase (HRP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. Horseradish peroxidase-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.