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Product Catalog
Bcl-2 (P65) Antibody
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Catalogue No : BS1511
| Host | Reactivity | Size | Application | stock | price | cart |
| BS1511 | H,M,R | 100μg | WB IHC IF |  |
218.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
BCL-2 (P65) antibody detects endogenous levels of BCL-2 protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of BCL-2 (P65) antibody in extracts from K562 cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of BCL-2 (P65) antibody in paraffin-embedded human colon carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Apoptosis defines a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kD,a membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.