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Product Catalog
BAD (R149) Antibody
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Catalogue No : BS1028
| Host | Reactivity | Size | Application | stock | price | cart |
| BS1028 | H,M,R | 200μg | WB IHC |  |
218.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
BAD (R149) antibody detects endogenous levels of BAD protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of BAD (R149) antibody in extracts from 293 cells treated with forskolin.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of BAD (R149) antibody in paraffin-embedded human breast carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.