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Product Catalog
PI3-kinase p85-α/γ(Tyr467/199) pAb
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Catalogue No : AP0230
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,(R) | 100UL | WB,IHC |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Molecular Weight
85
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
PI3-kinase p85-α/γ(Tyr467/199) pAb detects endogenous levels of PI3-kinase p85-α/γ protein.
Applications (Recommended Dilutions)
WB: 1:500-1:1000 IHC:1:50-1:200
Western Blot(WB)
Western blot (WB) analysis of PI3-kinase p85-α/γ(Tyr467/199) pAb in extracts from Hela cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analysis of PI3-kinase p85-α/γ(Tyr467/199)pAb in paraffin-embedded human breast carcinoma tissue
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.