Email:shdiahds@163.com
Phone:+1-909-839-7620
Fax:+1-909-839-7620
Click here for qusetions for this product!
Product Catalog
A-Raf (D297) pAb
Current Country :
Download PDF Datasheet
Catalogue No : BS1508
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC |  |
238.00 |  |
|
Contact information
|
||||||
Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 68 kDa
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
A-RAF (D297) pAb detects endogenous levels of A-RAF protein.
Applications (Recommended Dilutions)
WB: 1:500~1:1000 IHC: 1:50~1:200
Western Blot(WB)
Western blot (WB) analysis of A-RAF (D297) pAb in extracts from HeLa cells treated with PMA 125ng/ml 30'.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of A-RAF (D297) pAb in paraffin-embedded human breast carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.