Email:shdiahds@163.com
Phone:+1-909-839-7620
Fax:+1-909-839-7620
Click here for qusetions for this product!
Product Catalog
4E-BP1 (R51) Antibody
Current Country :
Download PDF Datasheet
Catalogue No : BS5549
| Host | Reactivity | Size | Application | stock | price | cart |
| BS5549 | H,M,R | 100μg | IHC |  |
258.00 |  |
|
Contact information
|
||||||
Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
4E-BP1 (R51) antibody detects endogenous levels of 4E-BP1 protein.
Applications (Recommended Dilutions)
IHC: 1:50~1:200
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of 4E-BP1 (R51) antibody in paraffin-embedded human lung adenocarcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.