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Product Catalog
14-3-3 ζ (V52) pAb
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Download PDF Datasheet
Catalogue No : BS1001
| Host | Reactivity | Size | Application | stock | price | cart |
| Rabbit | H,M,R | 100μg | WB IHC IP IF |  |
258.00 |  |
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Contact information
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Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Molecular Weight
~ 28 kDa
Purification & Purity
The pAb was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
14-3-3 zeta pAb detects endogenous levels of 14-3-3 zeta protein.
Applications (Recommended Dilutions)
IHC: 1:50~1:200 WB: 1:500~1:1000 IP: 1:50~1:200 IF: 1:50~1:200
Western Blot(WB)
Western blot (WB) analyzes of 14-3-3 ζ (V52) pAb in extracts from 293 Forskolin 40nM 30' NIH/3T3 PMA 250ng/ml 5' cells.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC) analyzes of 14-3-3 ζ (V52) pAb in paraffin-embedded human breast carcinoma tissue.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.